E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis

• DOI: 10.3390/microorganisms11081915
• Publication Date: 2023-07-28T05:56:16Z
• Authors: Bárbara Simas Chagas; Elias Tibúrcio Jú...; Ruany Cristyne de...; Daffany Luana dos...; Marconi Rego Barr...; Rita de Cássia Pe...; Maria da Conceiçã...; Vanessa Emanuelle...; Pedro Luiz França...; Antônio Humberto ...; Jacinto Costa Sil...; Marcus Vinícius d...; Antonio Carlos de...
• Keywords: Oncogene Proteins

Cervical cancer is associated with persistent infections by high-risk Human Papillomavirus (HPV) types that may have nucleotide polymorphisms and, consequently, different oncogenic potentials. Therefore, this study aimed to evaluate the genetic variability and structural effects of E7 oncogene HPV58 in cervical scraping samples from Brazilian women. The was developed patients hospitals metropolitan area Recife, PE, Brazil. most frequent HPV were, descending order abundance, HPV16, 31, 58. Phylogenetic analysis demonstrated isolates were classified into sublineages A2, C1, D2. Two positively selected mutations found E7: 63G 64T. G41R, G63D, T64A protein reduced stability structure. Utilizing an NF-kB reporter assay, we observed a decrease NK-kB pathway activity HPV58-E7 variant 54S compared WT E7. other detected variants presented similar In study, it possible identify interfere molecular interaction between viral oncoproteins host proteins.