Barley Yellow Dwarf Virus Influences Its Vector’s Endosymbionts but Not Its Thermotolerance

• DOI: 10.3390/microorganisms12010010
• Publication Date: 2023-12-19T16:17:24Z
• Authors: Evatt Chirgwin; Qiong Yang; Paul A. Umina; Joshua A. Thia; Alex Gill; Wei Song; Xinyue Gu; Perran A. Ross; Shu-Jun Wei; Ary A. Hoffmann
• Keywords: Rhopalosiphum padi; Buchnera; Barley yellow dwarf

The barley yellow dwarf virus (BYDV) of cereals is thought to substantially increase the high-temperature tolerance its aphid vector, Rhopalosiphum padi, which may enhance transmission efficiency. This based on experiments with North American strains BYDV and R. padi. Here, we independently test these by measuring temperature tolerance, via Critical Thermal Maximum (CTmax) knockdown time, Australian padi infected a local isolate. We further consider interaction between transmission, primary endosymbiont (Buchnera aphidicola), transinfected secondary (Rickettsiella viridis) reduces thermotolerance other species. failed find an in high temperatures BYDV-infected aphids or impact Rickettsiella thermotolerance. However, interacted endosymbionts unexpected ways, suppressing density Buchnera Rickettsiella. was also fourfold higher Rickettsiella-infected aphids. Our findings indicate that does not necessarily vector potential, at least for genotype combinations tested here. interactions suggest new ways influence how spreads, needs testing field context.