Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

• DOI: 10.3390/microorganisms12030597
• Publication Date: 2024-03-18T10:58:39Z
• Authors: Natalia S. Egozarian; Elena V. Emelyanova; Nataliya E. Suzina; Olesya I. Sazonova; Valentina N. Poli...; Tatiana O. Anokhina; Yonghong Wu; Inna P. Solyanikova
• Keywords: dormancy; QH301-705.5; <i>Rhodococcus opacus</i> strain 1CP; immobilization; phenol; box-PCR; Biology (General); Article; degradation

Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol is a toxic compound for living cells, so many bacteria degrade phenol in relatively low concentrations, up to 0.75 g L−1. The Rhodococcus opacus strain 1CP is an effective destructor of a wide range of pollutants. In the absence of a carbon source in the medium, cells of the R. opacus 1CP strain easily form cyst-like resting cells (CLC). The purpose of this work was to evaluate the viability of cells during long-term storage and the efficiency of the process of phenol destruction by R. opacus 1CP cells germinating after dormancy. Resting cells were obtained by simple cultivation in a rich medium followed by storage under static conditions. This is a simple approach to obtain a large amount of biomass. Decomposition of phenol proceeded via catechol followed by ortho-cleavage of aromatic ring. The induction of three phenol hydroxylases was detected by RT-PCR in cells germinated in a mineral medium with phenol as the carbon source. The stability of the genome of cells germinating after dormancy is shown by box-PCR. Dormant R. opacus 1CP cells, both suspended and immobilized, can be directly used for the decomposition of phenol after 4–12 months storage. In addition to phenol, after 9 months of storage, immobilized germinating cells easily metabolized 4-chlorophenol and 2,4,6-trichlorophenol. The results demonstrate a potential and simple approach toward achieving long-term storage of cells for further use in bioremediation.