Diversity and Bioactive Potential of Actinobacteria Isolated from a Coastal Marine Sediment in Northern Portugal

• DOI: 10.3390/microorganisms8111691
• Publication Date: 2020-10-31T01:34:47Z
• Authors: Inês Ribeiro; Mariana Girão; Diogo A. M. Alexa...; Tiago Ribeiro; Chiara Santos; Filipe Pereira; Ana P. Mucha; Ralph Urbatzka; Pedro N. Leão; Maria F. Carvalho
• Keywords: 0301 basic medicine; Natural products; secondary metabolism; antimicrobial activity; Cytotoxic activity; natural products; QH301-705.5; Coastal sediment; Antimicrobial activity; coastal sediment; Article; Actinobacteria; 03 medical and health sciences; 14. Life underwater; Secondary metabolism; Biology (General); cytotoxic activity

Natural compounds have had increasing applications in the biotechnological sector, with a large fraction of these substances being channeled to pharmaceutical industry due their important pharmacological properties. The discovery new bioactive molecules novel mechanisms action constitutes promising solution for design alternative therapeutic solutions. Actinobacteria are group morphologically and physiologically diverse bacteria well known production biotechnologically relevant compounds. Portuguese coast is scantly explored terms diversity respective potential, offering good opportunity find taxa natural products. In this study, we investigated associated sediment sample collected from intertidal zone beach northern Portugal, through cultivation-dependent approach, screened its antimicrobial cytotoxic potential. A total 52 strains were recovered marine sediment, largest isolates belonging genus Micromonospora. Bioactivity screening assays identified crude extracts six Streptomyces active against C. albicans, exhibiting minimum inhibition concentration (MIC) values range 3.90-125 μg mL-1. Twenty-five (obtained genera Micromonospora, Actinomadura) exhibited significant effects on viability at least one tested cancer cell line (breast ductal carcinoma T-47D liver hepatocellular HepG2). demonstrating activity and/or subjected metabolomic analysis (Mass spectrometry (MS)-based dereplication molecular networking analyses), indicating presence four clusters that may represent results obtained demonstrate importance bioprospecting underexplored environments, like coast, enhancing products, call attention relevance preserving genetic coastal environments.