Detection of Oxazolidinone Resistance Genes and Characterization of Genetic Environments in Enterococci of Swine Origin, Italy

• DOI: 10.3390/microorganisms8122021
• Publication Date: 2020-12-17T15:42:47Z
• Authors: Simona Fioriti; Gianluca Morroni; Sonia Nina Coccitto; Andrea Brenciani; Alberto Antonelli; Vincenzo Di Pilato; Ilaria Baccani; Simona Pollini; Lucilla Cucco; Alessandra Morelli; Marta Paniccià; Chiara Francesca ...; Gian Maria Rossolini; Eleonora Giovanetti
• Keywords: Linezolid; Enterococcus faecalis; Enterococcus faecium

One hundred forty-five florfenicol-resistant enterococci, isolated from swine fecal samples collected 76 pig farms, were investigated for the presence of optrA, cfr, and poxtA genes by PCR. Thirty Enterococcus isolates had at least one linezolid resistance gene. optrA was found to be most widespread gene (23/30), while cfr detected in 6/30 7/30 enterococcal isolates, respectively. WGS analysis also showed cfr(D) faecalis (n = 2 isolates) avium 1 isolate). The hybridized both on chromosome plasmids ranging ~25 ~240 kb. Twelve able transfer enterococci recipient. displayed a great variability genetic contexts identical or related transposons (Tn6628 Tn6674), (pE035 pWo27-9), chromosomal regions. environments identities with Tn6644-like transposon region p12-2300 plasmid; corresponding plasmid 4 faecium E8014; always Tn6657. Circular forms obtained only optrA- poxtA-carrying contexts. Clonality revealed E. (ST16, ST27, ST476, ST585) (ST21) clones previously humans. These results demonstrate dissemination origin Central Italy confirm spread animal settings.