Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property

• DOI: 10.3390/molecules22050844
• Publication Date: 2017-05-23T05:47:33Z
• Authors: Chang-Hao Cui; Da Jung Kim; Suk-Chae Jung; Sun-Chang Kim; Wan-Taek Im
• Keywords: HeLa

Minor ginsenosides, such as compound K, Rg3(S), which can be produced by deglycosylation of ginsenosides Rb1, showed strong anti-cancer effects. However, the anticancer effects gypenoside LXXV, is one deglycosylated shapes ginsenoside still unknown due to rarity its content in plants. Here, we cloned and characterized a novel ginsenoside-transforming β-glucosidase (BglG167b) derived from Microbacterium sp. Gsoil 167 efficiently hydrolyze XVII into applied it production LXXV at gram-scale with high specificity. In addition, activity was investigated against three cancer cell lines (HeLa, B16, MDA-MB231) vitro. Gypenoside significantly reduced viability, displaying an enhanced effect compared Rb1. Taken together, this enzymatic method would useful preparation for functional food pharmaceutical industries.