Anthocyanins Isolated from Vitis coignetiae Pulliat Enhances Cisplatin Sensitivity in MCF-7 Human Breast Cancer Cells through Inhibition of Akt and NF-κB Activation

• DOI: 10.3390/molecules25163623
• Publication Date: 2020-08-10T13:04:16Z
• Authors: Anjugam Paramanan...; Min Jeong Kim; Eun Joo Jung; Hye Jung Kim; Seong-Hwan Chang; Jin-Myung Jung; Soon Chan Hong; Sung Chul Shin; Gon Sup Kim; Won Sup Lee
• Keywords: MCF-7; XIAP; Viability assay; IκBα

Anthocyanins isolated from Vitis coignetiae Pulliat (Meoru in Korea) (AIMs) have various anti-cancer properties by inhibiting Akt and NF-κB which are involved drug resistance. Cisplatin (CDDP) is one of the popular agents. Studies reported that MCF-7 human breast cancer cells high resistance to CDDP compared other cell lines. In this study, we confirmed tested whether AIMs can overcome cells. Cell viability assay revealed were more resistant treatment than MDA-MB-231 exhibiting aggressive stem phenotype. significantly augmented efficacy with synergistic effects on Molecularly, Western blot analysis strongly increased moderately reduced p-NF-κB p-IκB inhibited CDDP-induced activation, reduction addition, downregulated an anti-apoptotic protein, XIAP, PARP-1 cleavage CDDP-treated Moreover, under TNF-α treatment, inhibition activation conclusion, enhanced sensitivity activity show relative intrinsic