A Multimethodological Characterization of Cannabis sativa L. Inflorescences from Seven Dioecious Cultivars Grown in Italy: The Effect of Different Harvesting Stages

• DOI: 10.3390/molecules26102912
• Publication Date: 2021-05-14T14:54:22Z
• Authors: Mattia Spano; Giacomo Di Matteo; Cinzia Ingallina; Bruno Botta; Deborah Quaglio; Francesca Ghirga; Silvia Balducci; Silvia Cammarone; Enio Campiglia; Anna Maria Giusti; Giuliana Vinci; Mattia Rapa; Salvatore Ciano; Luisa Mannina; Anatoly P. Sobolev
• Keywords: Isoleucine

The chemical profile of the female inflorescence extracts from seven Cannabis sativa L. dioecious cultivars (Carmagnola, Fibranova, Eletta Campana, Antal, Tiborszallasi, Kompolti, and Tisza) was monitored at three harvesting stages (4, 14, 30 September), reaching beginning flowering to end flowering/beginning seed formation, using untargeted nuclear magnetic resonance (NMR) targeted (ultra-high-performance liquid chromatography (UHPLC) spectrophotometry) analyses. tetrahydrocannabinol content always below legal limits (<0.6%) in all analyzed samples. NMR metabolite (sugars, organic acids, amino minor compounds) subjected principal components analysis (PCA) showed a strong variability according stages: samples harvested stage I were characterized by high sucrose myo-inositol, whereas ones II levels succinic acid, alanine, valine, isoleucine, phenylalanine, threonine. Samples III glucose, fructose, choline, trigonelline, malic formic some acids. ratio between chlorophylls carotenoids indicated that plants grew up exposed sun, Campana cultivar having highest pigment amount. Tiborszallasi polyphenol content. antioxidant activity generally observed II. All these results suggested inflorescences each hemp presented peculiar affected stage. This information could be useful for producers industries harvest appropriate obtain with suitable proper applications.