Development and Characterization of Solid Lipid Nanoparticles Loaded with a Highly Active Doxorubicin Derivative

• DOI: 10.3390/nano8020110
• Publication Date: 2018-02-20T08:54:22Z
• Authors: Barbara Stella; Elena Peira; Chiara Dianzani; Marina Gallarate; Luigi Battaglia; Casimiro Luca Gig...; Elena Boggio; Umberto Dianzani; Franco Dosio
• Keywords: Solid lipid nanoparticle; Characterization; Derivative (finance)

Solid lipid nanoparticles (SLNs) comprise a versatile drug delivery system that has been developed for the treatment of variety diseases. The present study will investigate feasibility entrapping an active doxorubicin prodrug (a squalenoyl-derivative) in SLNs. derivative-loaded SLNs are spherically shaped, have mean diameter 300–400 nm and show 85% w/w entrapment efficiency. effects on cell growth loaded SLNs, free examined using cytotoxicity colony-forming assays both human ovarian cancer line A2780 wild-type doxorubicin-resistant cells. Further assessments as to treatment’s ability induce death by apoptosis carried out analyzing annexin-V staining activation caspase-3. vitro data demonstrate squalenoyl-doxorubicin derivative increases its cytotoxic activity, well effect. This effect was particularly evident