Oral Supplementation of the Vitamin D Metabolite 25(OH)D3 Against Influenza Virus Infection in Mice

• DOI: 10.3390/nu12072000
• Publication Date: 2020-07-06T07:19:27Z
• Authors: Hirotaka Hayashi; Masatoshi Okamatsu; Honami Ogasawara; Naoko Tsugawa; Norikazu Isoda; Keita Matsuno; Yoshihiro Sakoda
• Keywords: Proinflammatory cytokine

Vitamin D is a fat-soluble vitamin that metabolized by the liver into 25-hydroxyvitamin [25(OH)D] and then kidney 1,25-dihydroxyvitamin [1,25(OH)2D], which activates receptor expressed in various cells, including immune for an overall immunostimulatory effect. Here, to investigate whether oral supplementation of D3 [25(OH)D3], major form metabolite 25(OH)D, has prophylactic effect on influenza A virus infection, mice were fed diet containing high dose 25(OH)D3 challenged with virus. In lungs 25(OH)D3-fed mice, viral titers significantly lower than standardly mice. Additionally, proinflammatory cytokines IL-5 IFN-γ downregulated after infection while anti-inflammatory not upregulated. These results indicate suppresses production inflammatory reduces replication clinical manifestations mouse model.