Ameliorative Effects of Daphnopsis costaricensis Extract against Oxazolone-Induced Atopic Dermatitis-like Lesions in BALB/c Mice

• DOI: 10.3390/nu14214521
• Publication Date: 2022-10-28T02:36:17Z
• Authors: Yunji Bae; Taeyoung Kim; Nojune Park; Sangho Choi; Dongkeun Yi; Silvia Soto; Nelson Zamora; Sunam Kim; Minhye Yang
• Keywords: Mice, Inbred BALB C; 0303 health sciences; Plant Extracts; Oxazolone; Immunoglobulin E; Article; Dermatitis, Atopic; 3. Good health; Mice; 03 medical and health sciences; <i>Daphnopsis costariensis</i>; atopic dermatitis; anti-inflammatory; 7,8-dimethoxyflavone; 7,2’-dimethoxyflavone; interleukin 4; β-hexosaminidase; Thymelaeaceae; Dinitrochlorobenzene; Animals; Cytokines; Mast Cells; Skin

The genus Daphnopsis has been traditionally used as a purgative, diuretic, stimulant, and psoriasis treatment. In this study, the anti-AD (atopic dermatitis) activities of the Daphnopsis costaricensis EtOH extract (DCE) were investigated in an oxazolone (OX)-induced mouse model of AD, and the anti-inflammatory effects of its active compounds were confirmed in PI-sensitized or IgE/DNP-BSA-sensitized RBL-2H3 cells. DCE improved the symptoms of OX-induced inflammatory dermatitis (swelling, erythema, and increased ear thickening) in OX-induced BALB/c mice ears and reduced epidermal thickness and mast cell infiltration. Eleven flavonoid compounds were isolated from DCE, and two compounds (7,8-dimethoxyflavone and 7,2’-dimethoxyflavone) significantly inhibited IL-4 overexpression in PI-induced RBL-2H3 cells and mast cell degranulation in IgE + DNP-BSA-induced RBL-2H3 cells. Our study indicates that DCE and two compounds (7,8-dimethoxyflavone and 7,2’-dimethoxyflavone) might effectively improve inflammatory and atopic skin symptoms.