Anti-Inflammatory Effect of Angelica gigas via Heme Oxygenase (HO)-1 Expression

• DOI: 10.3390/nu7064862
• Publication Date: 2015-06-15T14:57:58Z
• Authors: Joon Cho; Jung Kwon; Youngmi Cho; Inhye Kim; Se Kang
• Keywords: 0303 health sciences; Dose-Response Relationship, Drug; Plant Extracts; Communication; Anti-Inflammatory Agents; Membrane Proteins; Nitric Oxide; Antioxidants; Muscle, Smooth, Vascular; 3. Good health; Butyrates; Mice; 03 medical and health sciences; RAW 264.7 Cells; Gene Expression Regulation; Glucosides; Coumarins; Animals; Benzopyrans; Heme Oxygenase-1; Angelica

Angelica gigas (AG) is effective against various medical conditions such as bacterial infection, inflammation, and cancer. It contains a number of coumarin compounds and the group of interest is the pyranocoumarin, which comprises decursin and decursinol angelate. This group has an effect on controlling inflammation, which is caused by excessive nitric oxide (NO) production. Heme oxygenases (HOs), particularly HO-1, play a role in regulating the production of NO. Thus, this study aimed to investigate the anti-inflammatory effects of AG by measuring HO-1 expression. Treatments with CH2Cl2 layer and Angelica gigas extract (AGE) showed the highest NO inhibition effects. Decursin, decursinol angelate, and nodakenin were isolated from the CH2Cl2 layer of AGE. Decursin also demonstrated the highest anti-oxidative effect among the coumarins. Although decursin had the best NO inhibition and anti-oxidative effects, the effects of AGE treatment far surpassed that of decursin. This is owing to the combination effect of the coumarins present within AGE, which is a solvent extract of AG. The expression of HO-1 is an effective indicator of the anti-inflammatory effects of AG. Based on the results of the coumarin compounds, HO-1 expression was found to be dose dependent and specific to decursin.