Pantoea ananatis, A New Bacterial Pathogen Affecting Wheat Plants (Triticum L.) in Poland

• DOI: 10.3390/pathogens9121079
• Publication Date: 2020-12-22T01:42:01Z
• Authors: Krzysztof Krawczyk; Beata Wielkopolan; Aleksandra Obrępa...
• Keywords: rpoB; Pantoea

Wheat (Triticum aestivum) is one of the most economically important crops in world. During routine monitoring wheat pest, cereal leaf beetle (CLB, Oulema melanopus, Coleoptera, Chrysomelidae), Greater Poland region, it was observed that some leaves wounded by CLB also displayed brownish lesions with clear margins and yellow halo, disease symptoms resembling a bacterial infection. The aim this study therefore to investigate those establish causal agent disease. identification based on results Biolog’s Gen III system, 16S rRNA, gyrB genes sequencing, revealed presence eight strains Pantoea ananatis bacteria. Four were derived from (Ta024, Ta027, Ta030, Ta046), four CLB’s oral secretion (OUC1, OUD2, OUF2, OUG1). They shared nucleotide identity ranging 99 100% P. deposited GenBank database. Additionally, multi-locus sequence analysis (MLSA) concatenated sequences partial atpD, fusA, gyrB, rplB, rpoB performed. All isolated Poland, grouped into cluster supported high bootstrap value. Pathogenicity tests performed varieties plants have identified as To our knowledge, first report affecting plants.