Ultrasound Assisted Extraction of Saponins from Hedera helix L. and an In Vitro Biocompatibility Evaluation of the Extracts

• DOI: 10.3390/ph15101197
• Publication Date: 2022-09-28T22:53:19Z
• Authors: Adina I. Gavrila; Rodica Tatia; Ana-Maria Seciu-G...; Isabela Tarcomnicu; Cristina Negrea; Ioan Calinescu; Christina Zalaru; Lucia Moldovan; Anca D. Raiciu; Ioana Popa
• Keywords: Hedera helix; Biocompatibility

The aim of this study was to establish the best ultrasound assisted extraction (UAE) conditions saponins from Hedera helix L. leaves and evaluate in vitro biocompatibility extracts richest saponins. Different parameters, such as time, temperature, power, solvent plant material ratio, concentration, were investigated. most efficient a temperature 50 °C, an amplitude 40%, time 60 min, ratio 1:20 (w:v), 80% ethanol solvent. In cytotoxicity their influence on DNA content L929 (NCTC) fibroblasts tested. Until 200 µg/mL, studied cytocompatible with fibroblast cell lines at 48 h treatment. These culture results provide useful information for further applications pharmaceutical field.