Outlining the Phytoconstituents of Greek Clover Herb Extract and Assessment of Its Effect against Foodborne Infections Caused by Salmonella typhimurium

• DOI: 10.3390/ph17020259
• Publication Date: 2024-02-19T15:40:15Z
• Authors: Jawaher Alqahtani; Walaa A. Negm; Engy Elekhnawy; Moneerah J. Alqah...; Ehssan Moglad; Sarah Ibrahim; Suzy A. El-Sherbeni
• Keywords: Trigonella; Membrane permeability; Phytotherapy; Pathogenic bacteria

Owing to the spread of resistance between pathogenic bacteria, searching for novel compounds with antibacterial activity is essential. Here, we investigated potential Greek clover or Trigonella foenum-graecum herb extract on Salmonella typhimurium clinical isolates. The chemical profile was initially determined using LC-ESI-MS/MS, which explored 36 different compounds. Interestingly, fenugreek possessed action in vitro minimum inhibitory concentrations 64 512 µg/mL. mechanism studied by elucidating effect membrane properties S. including inner and outer permeability integrity. Remarkably, had detrimental effects 40–60% Moreover, vivo a gastrointestinal infection model bacteria. (200 mg/kg) improved outcomes tested mice. This represented noteworthy decrease (p < 0.05) bacterial count small intestine caecum tissues. survival rate fenugreek-extract-treated mice significantly increased compared typhimurium-infected group. Additionally, there an improvement histological immunohistochemical features tumor necrosis factor-alpha. In addition, ELISA qRT-PCR, proinflammatory oxidative stress markers Consequently, should be further other food pathogens.