Intracellular Accumulation and Secretion of YKL-40 (CHI3L1) in the Course of DMSO-Induced HL-60 Cell Differentiation

• DOI: 10.3390/ph17040443
• Publication Date: 2024-03-31T17:42:30Z
• Authors: Izabela Jatczak-P...; Alicja Ewiak-Pasz...; Małgorzata Domowicz; Anna Jurewicz; Mariusz Stasiołek
• Keywords: RS1-441; cell differentiation; YKL-40 (CHI3L1); 0303 health sciences; 03 medical and health sciences; Pharmacy and materia medica; R; neutrophil; Medicine; DMSO; HL-60 cells; Article

YKL-40 (CHI3L1) is a matrix glycoprotein stored in human neutrophil-specific granules and released upon activation. While it implicated inflammation, cancer progression, cell differentiation, its exact physiological role remains unclear. This study investigated the intracellular expression secretion of by untreated DMSO-treated HL-60 cells association with surface CD11b CD66b throughout differentiation process (up to 120 h). Secreted protein mRNA levels YKL-40, CD66b, were measured ELISA quantitative RT-PCR, respectively. The assessed flow cytometry. A significant increase confirmed DMSO-induced cells. Upon DMSO stimulation, increased time-dependent manner, unlike CD66b. lack (a granulocyte maturation activation marker) on might suggest that treatment did not induce full or was increasing up 96 h then declined. into culture medium detectable only at later time points (96 h), which correlated decreased proliferation These findings sequential changes production during contribute better understanding YKL-40’s involvement both processes disease development, including multiple sclerosis.