Exploring Microbial Metabolite Receptors in Inflammatory Bowel Disease: An In Silico Analysis of Their Potential Role in Inflammation and Fibrosis

• DOI: 10.3390/ph17040492
• Publication Date: 2024-04-12T13:28:06Z
• Authors: Michail Spathakis; Nikolas Dovrolis; Eirini Filidou; Leonidas Kandilog...; Gesthimani Tarapatzi; Vassilis Valatas; Ioannis Drygiannakis; Vasilis Paspaliaris; Konstantinos Arva...; Vangelis G. Manol...; George Kolios; Stergios Vradelis
• Keywords: RS1-441; 0301 basic medicine; 03 medical and health sciences; Pharmacy and materia medica; inflammatory bowel disease; inflammation; microbiota; R; metabolite receptors; Medicine; metabolites; Article

Metabolites produced by dysbiotic intestinal microbiota can influence disease pathophysiology participating in ligand–receptor interactions. Our aim was to investigate the differential expression of metabolite receptor (MR) genes between inflammatory bowel (IBD), healthy individuals (HIs), and controls order identify possible interactions with fibrotic pathways intestine. RNA-sequencing datasets containing 643 Crohn’s (CD) patients, 467 ulcerative colitis (UC) patients 295 HIs, 4 Campylobacter jejuni-infected were retrieved from Sequence Read Archive, performed using RaNA-seq online platform. The identified differentially expressed MR used for correlation analysis up- downregulated IBD, as well functional enrichment a R based pipeline. Overall, 15 exhibited dysregulated IBD. In inflamed CD, hydroxycarboxylic acid receptors 2 3 (HCAR2, HCAR3) upregulated associated recruitment innate immune cells, while, non-inflamed CD ileum, cannabinoid 1 (CNR1) sphingosine-1-phospate (S1PR4) involved regulation B-cell activation. UC, HCAR2 HCAR3 more closely process TH-17 cell differentiation, while pregnane X (NR1I2) transient potential vanilloid (TRPV1) epithelial barrier maintenance. results elucidate landscape highlighting associations disease-related functions that could guide development new targeted therapies.