Antimicrobial Activity of Ca-Alginate/Chitosan Nanocomposite Loaded with Camptothecin

• DOI: 10.3390/polym13203559
• Publication Date: 2021-10-18T03:25:15Z
• Authors: Wafa Al-Gethami; Noha Al-Qasmi
• Keywords: Nanomaterials; Camptothecin; Klebsiella pneumonia

The main aim of this study was to prepare antimicrobial nanocomposites consisting alginate, chitosan, and camptothecin (CPT). CPT-loaded calcium alginate (Ca-Alg2) alginate/chitosan (Ca-Alg2-CH) nanomaterials were synthesized characterized using infrared (IR) spectroscopy, X-ray diffraction (XRD), UV-Vis scanning electron microscopy (SEM). activity the genetic effects Ca-Alg2/CPT Ca-Alg2-CH/CPT on Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia studied. repetitive element polymerase chain reaction analysis technique used assess changes in bacterial material due processing nanomaterials. results showed presence a strong chemical interaction between CPT loaded successfully both Furthermore, test that nanocomposite susceptible S. E. K. pneumonia; other hand, more coli resistant aureus. less efficient than killing Gram-negative treated bacteria. Moreover, revealed PCR polymorphic banding pattern. This observation provides an excellent guide ability some polymers induce point mutations DNA.