Polyethylenimine-Modified Bombyx mori Silk Fibroin as a Delivery Carrier of the ING4-IL-24 Coexpression Plasmid

• DOI: 10.3390/polym13203592
• Publication Date: 2021-10-21T01:31:26Z
• Authors: Longxing Niu; Guo Chen; Yanfei Feng; Xueping Liu; Peng Pan; Linling Huang; Ying Guo; Mingzhong Li
• Keywords: Polyethylenimine; Fibroin; Zeta potential

One of the major challenges for lung cancer gene therapy is to find a delivery vector with high efficiency and low toxicity. In this study, low-molecular-weight polyethyleneimine (PEI, 1.8 kDa) was grafted onto side chains Bombyx mori silk fibroin (BSF) prepare cationized BSF (CBSF), which used package plasmid DNA (pDNA) encoded by inhibitor growth 4 (ING4) interleukin-24 (IL-24). FTIR 1H-NMR spectra demonstrated that PEI effectively coupled amino bonds. The results trinitrobenzene sulfonic acid method zeta potential showed free group content on increased from 125.1 ± 1.2 µmol/mL 153.5 2.2 µmol/mL, isoelectric point 3.68 8.82, reversed - 11.8 0.1 mV + 12.4 0.3 after grafting. Positively charged CBSF could pDNA form spherical CBSF/pDNA complexes. vitro, human adenocarcinoma A549 cells embryonic fibroblast WI-38 were transfected Confocal laser scanning microscopy analysis flow cytometry tests complexes can transfect cells, transfection higher than 25 kDa PEI/pDNA CCK-8 assay significantly inhibited proliferation but had no significant effect exhibited lower cytotoxicity PEI. Therefore, system, constructed PEI-modified protein ING4-IL-24 double coexpression has applications in cancer.