Evaluation of Shifted Excitation Raman Difference Spectroscopy and Comparison to Computational Background Correction Methods Applied to Biochemical Raman Spectra

• DOI: 10.3390/s17081724
• Publication Date: 2017-07-27T15:28:56Z
• Authors: Eliana Cordero; Florian Korinth; Clara Stiebing; Christoph Krafft; Iwan Schie; Jürgen Popp
• Keywords: signal to noise ratio; Computers; Chemical technology; SERDS; TP1-1185; Raman spectroscopy; SERDS; EMSC; background correction; signal to noise ratio; Spectrum Analysis, Raman; 01 natural sciences; Article; 0104 chemical sciences; Spectrometry, Fluorescence; EMSC; Raman spectroscopy; background correction

Raman spectroscopy provides label-free biochemical information from tissue samples without complicated sample preparation. The clinical capability of has been demonstrated in a wide range vitro and vivo applications. However, challenge for applications is the simultaneous excitation auto-fluorescence majority tissues interest, such as liver, bladder, brain, others. bands are then superimposed on fluorescence background, which can be several orders magnitude larger than signal. To eliminate disturbing approaches available. Among instrumentational methods shifted difference (SERDS) widely applied studied. Similarly, computational techniques, instance extended multiplicative scatter correction (EMSC), have also employed to remove undesired background contributions. Here, we present theoretical experimental evaluation comparison removal spectra based SERDS EMSC.