Construction of a Biosensor Based on a Combination of Cytochrome c, Graphene, and Gold Nanoparticles

• DOI: 10.3390/s19010040
• Publication Date: 2018-12-24T15:37:49Z
• Authors: Chenxing Guo; Jianfang Wang; Xianzhe Chen; Yujiao Li; Lifang Wu; Jin Zhang; Cheng-an Tao
• Keywords: Chemical technology; graphene; Cytochromes c; Metal Nanoparticles; hydrogen peroxide; Oxides; direct electrochemistry; TP1-1185; Biosensing Techniques; Hydrogen Peroxide; 02 engineering and technology; biosensor; Article; Carbon; cytochrome c; Fluorocarbon Polymers; Limit of Detection; gold nanoparticles; Electrochemistry; Graphite; cytochrome <i>c</i>; 0210 nano-technology; Electrodes

A biosensor based on a combination of cytochrome c (Cyt c), electrochemical reduced graphene oxides (ERGO), and gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) was fabricated. The proposed biosensor electrode was denoted as GCE/ERGO-Nafion/AuNPs/Cyt c/Nafion, where ERGO-Nafion was deposited by dropping graphene oxides-Nafion mixed droplet first and following electrochemical reduction, AuNPs were directly deposited on the surface of the ERGO-Nafion modified electrode by electrochemical reduction, and other components were deposited by the dropping-dry method. The effect of the deposition amount of AuNPs on direct electrochemistry of Cyt c in the proposed electrode was investigated. The hydrogen peroxide was taken to evaluate the performance of the proposed biosensor. The results showed that the biosensor has great analytical performance, including a high sensitivity, a wide linear range, a low detection limit, and good stability, reproducibility, and reliability.