Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD

• DOI: 10.3390/s19204496
• Publication Date: 2019-10-17T15:07:59Z
• Authors: Sofia el Manouni ...; Sofie Bosch; Jesse Lemmen; Marina Brizzio Br...; Ibrahim Ayada; Alfian Wicaksono; James Covington; Marc Benninga; Nanne de Boer; Tim de Meij
• Keywords: Male; Adolescent; TP1-1185; Article; Gas Chromatography-Mass Spectrometry; Feces; 03 medical and health sciences; Crohn Disease; inflammatory bowel disease; 11. Sustainability; Humans; Child; Volatile Organic Compounds; 0303 health sciences; gas chromatography-ion mobility spectrometry; Chemical technology; QH; biomarkers; Inflammatory Bowel Diseases; 16. Peace & justice; 3. Good health; Case-Control Studies; Child, Preschool; Colitis, Ulcerative; Female; TD; Biomarkers; RC

Endoscopic evaluation is mandatory in establishing the diagnosis of pediatric inflammatory bowel disease (IBD), but unfortunately carries a high burden on patients. Volatile organic compounds (VOC) have been proposed as alternative, noninvasive diagnostic biomarkers for IBD. The current study aimed to assess and compare the potential of fecal and urinary VOC as diagnostic biomarkers for pediatric IBD in an intention-to-diagnose cohort. In this cohort study, patients aged 4–17 years, referred to the outpatient clinic of a tertiary referral center under suspicion of IBD, were eligible to participate. The diagnosis was established by endoscopic and histopathologic assessment, participants who did not meet the criteria of IBD were allocated to the control group. Participants were instructed to concurrently collect a fecal and urinary sample prior to bowel lavage. Samples were analyzed by means of gas chromatography–ion mobility spectrometry. In total, five ulcerative colitis patients, five Crohn’s disease patients, and ten age and gender matched controls were included. A significant difference was demonstrated for both fecal (p-value, area under the curve; 0.038, 0.73) and urinary (0.028, 0.78) VOC profiles between IBD and controls. Analysis of both fecal and urinary VOC behold equal potential as noninvasive biomarkers for pediatric IBD diagnosis.