Bioinformatics Analysis Confirms the Target Protein Underlying Mitotic Catastrophe of 4T1 Cells under Combinatorial Treatment of PGV-1 and Galangin

• DOI: 10.3390/scipharm89030038
• Publication Date: 2021-08-11T02:40:31Z
• Authors: Nurul Awali Fauzi...; Febri Wulandari; Eri Prasetyo Nugroho; Adam Hermawan; Edy Meiyanto
• Keywords: Galangin; Prometaphase; Mitotic catastrophe; Mitotic index

Pentagamavunon-1 (PGV-1), a potential chemopreventive agent with strong cytotoxic effect, modulates prometaphase arrest. Improvement to get higher effectiveness of PGV-1 is new challenge. A previous study reported that the natural compound, galangin, has antiproliferative activity against cancer cells lower cytotoxicity effect. This aims develop combinatorial treatment and galangin as an anticancer than single agent. In this study, 4T1, TNBC model cell, was treated combination galangin. As result, showed effect IC50 values 8 120 µM, respectively. Combining those chemicals synergistic impact, shown by index (CI) value 1. Staining May Grunwald-Giemsa reagent indicated mitotic catastrophe evidence, characterized micronuclear multinucleated morphology. Moreover, senescence percentage treatment. Furthermore, bioinformatics investigations target CDK1, PLK1, AURKB, overexpression proteins in are essential regulating cell cycle conclusion, exhibit be chemotherapeutic drug mechanism induction.