Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

• DOI: 10.3390/separations10010036
• Publication Date: 2023-01-06T08:31:28Z
• Authors: Josiane F. da Silva; Clara M. G. Lima; Débora L. da Silva; Ivonea S. do Nasc...; Sarah de O. Rodri...; Letícia A. Gonçalves; Renata F. Santana; Waseem Khalid; Silvani Verruck; Talha Bin Emran; Irwin R. A. de Me...; Henrique D. M. Co...; Mayeen U. Khandaker; Mohammad R. I. Fa...; Rafael da C. I. F...
• Keywords: Biomolecule

Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds. Cryogels, which are generated by freezing reactive polymerization mixture, highlighted due their versatility low relative cost as macroporous, polymeric, adsorbents. The conversion cryogels affinity adsorbents is one possible alternative optimal application. Some the most often utilized supports immobilizing particular ligands columns manufactured epoxy radicals on surfaces. purification biomolecules high degree specificity, such lectins glycoproteins an glycosylated groups, garnered in use fixed non-traditional beds functionalized interest. interaction both robust enough permit adsorption reversible dissociation molecules response changes solution’s pH. When compared other protein A-based approaches, this been shown be more advantageous than its counterparts terms ease use, cost-effectiveness. Information used chromatographic published explored.