Geographical Origin Authentication of Edible Chrysanthemum morifolium Ramat. (Hangbaiju) Using Stable Isotopes

• DOI: 10.3390/separations10050287
• Publication Date: 2023-05-04T05:33:36Z
• Authors: Hanyi Mei; Jing Nie; Shu Wang; Yongzhi Zhang; Chunlin Li; Shengzhi Shao; Shanshan Shao; Karyne M. Rogers; Yuwei Yuan
• Keywords: Chrysanthemum morifolium

Chrysanthemum morifolium Ramat., known as Hangbaiju (HBJ), is a high-value edible, medicinal product where the flowers are infused in hot water and drunk tea. Its quality efficacy closely related to its geographical origin. Consequently, it vulnerable fraudulent substitution by other lower-value products. In this study, cultivation (variety different growth stages) isotopic fractionation between flower, stem, leaf were studied. Samples from four HBJ varieties characterized using stable isotopes (δ13C, δ15N, δ2H, δ18O, %C, %N) across three producing regions Zhejiang province, China. The results showed that there no significant differences compositions for varieties, but plant tissues (flower, leaf, etc.). Furthermore, composition altered dramatically at stages. δ15N (r = 0.6809) δ2H 0.6102) correlations stems leaves (SL) (F) of relatively good, δ13C correlation 0.2636) SL F was weak, δ18O 0.01) had almost correlation. A supervised multivariate statistical model (partial least squares discriminant analysis, PLS-DA) used discriminate with high accuracy (66.7%, 66.7%, 100%, respectively). Our findings show combined analysis provide an effective method identification HBJ.