Decreased Ubiquitination and Acetylation of Histones 3 and 4 Are Associated with Obesity-Induced Disorders of Spermatogenesis in Mice

• DOI: 10.3390/toxics12040296
• Publication Date: 2024-04-17T12:22:44Z
• Authors: Mahamadou Fofana; Zhenyang Li; Han Li; Wenqi Li; Lu Wu; Lu Lu; Qizhan Liu
• Keywords: 0301 basic medicine; 0303 health sciences; high-fat diet; Chemical technology; TP1-1185; reproductive disorders; spermatogenesis; epigenetic; Article

Background: Obesity, a chronic metabolic disorder, is related to cardiovascular diseases, diabetes, cancer, and reproductive disorders. The relationship between obesity male infertility now well recognized, but the mechanisms involved are unclear. We aimed observe effect of on spermatogenesis investigate role histone ubiquitination acetylation modifications in obesity-induced Methods: Thirty C57BL/6J mice were randomly divided into two groups. control group was fed with general maintenance diet (12% fat), while high-fat (HFD) 40% fat for 10 weeks; then, they mated normal females. fertility calculated, testicular sperm morphology observed, expression levels key genes modification during detected. Results: number decreased, as motility, malformations increased. In testes, mRNA protein gonadotropin-regulated RNA helicase (GRTH/DDX25), chromosome region maintenance-1 (CRM1), high-mobility B2 (HMGB2), phosphoglycerate kinase 2 (PGK2), angiotensin-converting enzyme (tACE) decreased. Furthermore, led decrease ubiquitinated H2A (ubH2A) reduced H3 K18 (H3AcK18) H4 K5, K8, K12, K16 (H4tetraAck), which disrupted protamine 1 (Prm1) deposition testis tissue. Conclusion: These results suggest that low linked disorders spermatogenesis, contributing better understanding damage reproduction.