Cellular Uptake and Toxicological Effects of Differently Sized Zinc Oxide Nanoparticles in Intestinal Cells

• DOI: 10.3390/toxics9050096
• Publication Date: 2021-04-27T10:19:11Z
• Authors: Anna Mittag; Christian Hoera; Alexander Kämpfe; Martin Westermann; Jochen Kuckelkorn; Thomas Schneider; Michael Glei
• Keywords: Caco-2; Nanotoxicology

Due to their beneficial properties, the use of zinc oxide nanoparticles (ZnO NP) is constantly increasing, especially in consumer-related areas, such as food packaging and additives, which leading an increased oral uptake ZnO NP. Consequently, aim our study was investigate cellular two differently sized NP (<50 nm <100 nm; 12–1229 µmol/L) using human intestinal cell lines (Caco-2 LT97) examine possible resulting toxic effects. nm) were internalized by both led intracellular changes. Both caused time- dose-dependent cytotoxic effects, at concentrations 614 µmol/L 1229 µmol/L, associated with rate apoptotic dead cells. < 100 altered cycle LT97 cells but not that Caco-2 50 formation micronuclei The Ames test revealed no mutagenicity for Our results indicate potential toxicity after exposure, should be considered before application.