Proteomic Analysis of the Murine Liver Response to Oral Exposure to Aflatoxin B1 and Ochratoxin A: The Protective Role to Bioactive Compounds

• DOI: 10.3390/toxins17010029
• Publication Date: 2025-01-09T10:17:08Z
• Authors: Silvia Trombetti; Alessandra Cimbalo; Michela Grosso; Pilar Vila-Donat; Jordi Mañes; Lara Manyes
• Keywords: Ochratoxins; KEGG; Proteome

Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) are considered the most important mycotoxins in terms of food safety. The aim this study was to evaluate hepatotoxicity AFB1 OTA exposure Wistar rats assess beneficial effect fermented whey (FW) pumpkin (P) as functional ingredients through a proteomic approach. For experimental procedures, were fed individually or combination, with addition FW FW-P mixture during 28 days. proteomics analysis, peptides separated using LC-MS/MS-QTOF system differentially expressed proteins (DEPs) statistically filtered (p < 0.05) distinguishing males from females. Gene ontology visualization allowed identification involved biological processes such response xenobiotic stimuli liver development. Likewise, KEGG pathway analysis reported metabolic routes affected, followed by carbon metabolism biosynthesis amino acids. Overall, results highlighted strong downregulation DEPs presence but not both, suggesting synergistic effect. However, P have helped mitigation triggered mycotoxins.