Nephroprotective Role of Resveratrol and Ursolic Acid in Aristolochic Acid Intoxicated Zebrafish

• DOI: 10.3390/toxins7010097
• Publication Date: 2015-01-13T16:28:59Z
• Authors: Yu-Ju Ding; Chiao-Yin Sun; Chi-Chung Wen; Yau-Hung Chen
• Keywords: Ursolic acid; Aristolochic Acid; Nephrotoxicity

The nephrotoxicity of aristolochic acid (AA) is well known, but information regarding the attenuation AA-induced toxicity limited. aim present study was to nephroprotective effects resveratrol (Resv) and ursolic (UA) in a zebrafish model. We used two transgenic lines, Tg(wt1b:EGFP) Tg(gata1:DsRed), evaluate Resv UA by recording subtle changes kidney red blood cell circulation. Our results demonstrated that both treatment can attenuate malformations improve Glomerular filtration rate assays revealed restore renal function (100% for Mock; 56.1% ± 17.3% AA-treated; 80.2% 11.3% Resv+AA; 83.1% 8.1% UA+AA, n = 15). Furthermore, real-time RT-PCR experiments showed pre-treatment with either or suppresses expression pro-inflammatory genes. In conclusion, our findings reveal nephrotoxicities be attenuated UA. Therefore, we believe represent an efficient model screening AA-protective natural compounds.