Inhibition of Rabies Virus by 1,2,3,4,6-Penta-O-galloyl-β-d-Glucose Involves mTOR-Dependent Autophagy

• DOI: 10.3390/v10040201
• Publication Date: 2018-04-18T07:51:13Z
• Authors: Zhongzhong Tu; Wenjie Gong; Yan Zhang; Ye Feng; Yan Liu; Changchun Tu
• Keywords: PGG; IPS; RABV; antiviral; CVS-11; 0301 basic medicine; Rabies; IPS; Virus Replication; Microbiology; Antiviral Agents; Article; Mice; 03 medical and health sciences; Autophagy; CVS-11; Animals; 0303 health sciences; PGG; TOR Serine-Threonine Kinases; antiviral; Survival Analysis; QR1-502; Hydrolyzable Tannins; 3. Good health; Disease Models, Animal; RABV; Rabies virus; Sweetening Agents; Female

The compound 1,2,3,4,6-penta-O-galloyl-β-d-glucose (PGG), a gallotannin present in various plants such as Rhus chinensis Mill and Paeonia suffruticosa, has broad spectrum of antiviral effects. study investigated its potency against infection mice with rabies virus (RABV). Results demonstrated that PGG strongly inhibited titers (50-fold), viral mRNA expression (up to 90%), protein synthesis vitro. Importantly, we found not only suppressed adsorption entry, but also directly inactivated RABV through suppression autophagy by mediating activation the mTOR-dependent signaling pathway. In vivo, (10 mg/kg) alleviated clinical symptoms reduced mortality infected 27.3%. Collectively, our results indicate potent anti-RABV effect, merits further investigation an drug.