Full-Length Genomic RNA of Foot-and-Mouth Disease Virus Is Infectious for Cattle by Injection

• DOI: 10.3390/v14091924
• Publication Date: 2022-08-31T06:09:36Z
• Authors: Hanna Keck; Benedikt Litz; Bernd Hoffmann; Julia Sehl-Ewert; Martin Beer; Michael Eschbaumer
• Keywords: 2. Zero hunger; naked RNA; 0303 health sciences; single-stranded positive-sense RNA; foot-and-mouth disease virus; infectivity; Cattle Diseases; Genomics; Microbiology; QR1-502; Article; virology; 3. Good health; 03 medical and health sciences; foot-and-mouth disease virus; safe sample transport; single-stranded positive-sense RNA; TRIzol extraction; naked RNA; infectivity; RNA transfection; lipofectamine; self-transfection; BHK cells; Foot-and-Mouth Disease Virus; Foot-and-Mouth Disease; Animals; RNA, Viral; safe sample transport; Cattle; TRIzol extraction

Safe sample transport is of great importance for infectious diseases diagnostics. Various treatments and buffers are used to inactivate pathogens in diagnostic samples. At the same time, adequate preservation, particularly nucleic acids, essential allow an accurate laboratory diagnosis. For viruses with single-stranded RNA genomes positive polarity, such as foot-and-mouth disease virus (FMDV), however, naked full-length viral can itself be infectious. In order assess risk infection from inactivated FMDV samples, two animal experiments were performed. first trial, six cattle injected (isolate A22/IRQ/24/64) into tongue epithelium. All animals developed clinical within days was reisolated serum saliva second another group exposed by instilling it on spraying nose. The observed 10 after exposure. remained clinically unremarkable isolation well genome detection negative. No transfection reagent any inoculations. conclusion, infected injection RNA, but not non-invasive exposure RNA. Inactivated samples that contain carry only a negligible infecting animals.