CD4+ T cell activation promotes the differential release of distinct populations of nanosized vesicles

• DOI: 10.3402/jev.v1i0.18364
• Publication Date: 2012-04-19T14:49:54Z
• Authors: Els J. van der Vlist; Ger J.A. Arkesteijn; Chris H.A. van de...; Willem Stoorvogel; Esther N.M. Nolte...; Marca H.M. Wauben
• Keywords: Nano-sized vesicles, exosomes, microvesicles, microparticles, extracellular vesicles, flow cytometry, NTA, T cells, immune regulation; 0303 health sciences; 03 medical and health sciences; Original Research Article

Many cell types release nanosized vesicles derived from endosomal compartments (exosomes) or the plasma membrane. Vesicles actively released by CD4(+) T cells have immune-modulatory characteristics. Using our recently developed high-resolution flow cytometry-based method for analysis of individual vesicles, we here investigated how receptor (TCR)-triggering and co-stimulatory signals influence quantity characteristics cells. We found that number within buoyant density range characteristic exosomes (1.10-1.19 g/ml) was increased TCR-triggering additional had a potentiating effect on vesicle release. However, increase in varied substantially between fractions 1.10-1.19 g/ml highest populations 1.14 1.17 fractions. Heterogeneity also observed Based lipid bilayer fluorescent labelling intensity light scattering, 3 distinct subpopulations were identified. One subpopulation significantly more upon activation than other subpopulations, this dependent high levels co-stimulation. These data show heterogeneous population indicate differentially regulate depending their status.