Cellular stress conditions are reflected in the protein and RNA content of endothelial cell‐derived exosomes

• DOI: 10.3402/jev.v1i0.18396
• Publication Date: 2012-04-19T14:51:40Z
• Authors: Olivier G. de Jong; Marianne C. Verhaar; Yong Chen; Pieter Vader; Hendrik Gremmels; George Posthuma; Raymond M. Schiff...; Marjan Gucek; Bas W.M. van Balkom
• Keywords: 0301 basic medicine; 03 medical and health sciences; proteomics; tumor necrosis factor alpha; hypoxia; extracellular vesicles; exosomes; RNA; proteomics; hypoxia; tumor necrosis factor alpha; RNA; exosomes; Original Research Article; extracellular vesicles; 3. Good health

The healthy vascular endothelium, which forms the barrier between blood and surrounding tissues, is known to efficiently respond stress signals like hypoxia inflammation by adaptation of cellular physiology secretion (soluble) growth factors cytokines. Exosomes are potent mediators intercellular communication. Their content consists RNA proteins from cell origin, thus depends on condition these cells at time exosome biogenesis. It has been suggested that exosomes protect their target through transfer proteins. We hypothesized endothelium-derived involved in endothelial response stress, protein reflect effects induced hypoxia, or hyperglycemia.We exposed cultured different types (hypoxia, TNF-α-induced activation, high glucose mannose concentrations) compared mRNA produced microarray analysis a quantitative proteomics approach.We identified 1,354 1,992 mRNAs cell-derived exosomes. Several showed altered abundances after exposure producing were confirmed immunoblot qPCR analysis.Our data show activation reflected composition, sugar concentrations alters composition only minor extend, does not affect composition.