Comparison of methods for the extraction of DNA from formalin-fixed paraffin-embedded tissues for human identification

• DOI: 10.3408/jafst.734
• Publication Date: 2017-09-13T22:11:07Z
• Authors: Kohei Tomonari; Ayane Sonoda; Ami Ikehara; Seiki Nakao; Nao Mori; Takaaki Ono; Yoshito Tomisaka; Eiji Nishi
• Keywords: genomic DNA; DNA profiling; STR analysis

Formalin-fixed paraffin-embedded (FFPE) tissues are one of the most effective tools for human identification in forensic science. To evaluate effectiveness identification, we compared following six methods extraction DNA from FFPE tissues; EZ1 Investigator Kit, ReliaPrep gDNA Miniprep System, Isolator PS-Rapid Reagent, QIAamp Tissue NucleoSpin XS and phenol-chloroform method. extracted using each method two tissue blocks were quantified by three different quantification (Quant-it dsDNA HS Assay Kit quantitative PCR based on 207 bp 98 regions D17Z1 locus), short tandem repeat (STR) typing AmpFlSTR Identifiler Plus Amplification was conducted. All extracts highly degraded, large differences observed among total yields determined these methods. obtained had highest yield all In STR with 1 ng template estimated region locus, greatest number detected alleles Kit. contrast, Reagent inferior to other terms via typing. conclusion, methods, considering results this study, its cost-effectiveness, universal use