FEATURES OF INTESTINAL MICROBIOTA IN PATIENTS WITH NONALCOHOLIC FATTY LIVER DISEASE: EFFECTS ON MARKERS OF INFLAMMATION AND HEPATIC STEATOSIS

• DOI: 10.36740/wlek202203108
• Publication Date: 2022-04-29T06:51:52Z
• Authors: Yana V. Nikiforova; Galina D. Fadieienko; Alexey E. Gridnev; Inna E. Kushnir; Tatiana A. Solome...; Valentina M. Cher...; Olena G. Kurinna
• Keywords: Proinflammatory cytokine; Steatosis

The aim: To study the state of intestinal microbiota (ІМ) in patients with Nonalcoholic fatty liver disease (NAFLD) and to determine changes its composition at level basic phylotypes.Materials methods: included 114 NAFLD metabolic disorders 64 control group. Determination ІМ major phylotypes was performed by identifying total bacterial DNA Bacteroidetes, Firmicutes Actinobacteria quantitative polymerase chain reaction (PCR) real time (qRT-PCR) using universal primers for 16S rRNA gene taxon-specific production (Thermo Fisher Scientific).Results: It defined weak correlation between content proinflammatory markers (C-reactive protein (CRP) Tumor necrosis factor (TNF) alpha) (p <0.05) inverse CRP Bacteroidetes <0.001). Also have been observed significant main phyla direction increasing a high degree steatosis elevated levels cytokines <0.05).Conclusions: IM imbalance leads excessive synthesis pro-inflammatory cytokines, promotes activation cellular mechanisms, which increases flow acids into hepatocytes hepatic steatosis.