Involvement of PGC7 and UHRF1 in the regulation of DNA methylation of the IG-DMR in the imprinted <italic>Dlk1-Dio3</italic> locus

• DOI: 10.3724/abbs.2022080
• Publication Date: 2022-06-25T13:02:48Z
• Authors: Mengying Yu; Yingxiang Liu; Zhuo Han; Wei Du; Bingxue Chen; Lei Zhang; Hongni Xue; Zihan Zhang; Zekun Guo
• Keywords: Genomic Imprinting; Histone Methylation; Epigenomics; DNA methyltransferase; Cancer Epigenetics

The gene dosage at the imprinted Dlk1-Dio3 locus is critical for cell growth and development. A relatively high expression within region, especially active of Gtl2, has been identified as only reliable marker pluripotency. DNA methylation state IG-DNA methylated regions (DMR), which located upstream Gtl2 gene, dominantly contributes to control in locus. However, precise mechanism underlying regulation IG-DMR remains largely unknown. Here, we use F9 embryonal carcinoma line, a low pluripotent model, identify responsible IG-DMR, find that interaction PGC7 with UHRF1 involved maintaining inducing hypermethylation region. cooperatively bind regulate histones this promotes recruitment DNMT1 by maintain between strengthens their binding H3K9me3 leads further enrichment recruiting specific histone methyltransferase SETDB1. Consequently, abundance DNMT3A increases level In summary, propose new provide insight into understanding difference levels cells.