Association analysis between mixed exposure to phenols and semen quality

• DOI: 10.3724/sp.j.1123.2023.09009
• Publication Date: 2024-03-28T01:31:52Z
• Authors: Xiaoli SHEN; Weifeng TANG; Junxia LIU; Junjie AO; Xiaoning LIU; Xian HUANG; Jin QIU; Jun ZHANG; Qianlong ZHANG
• Keywords: Paraben; Semen quality

<p id="p00005">Phenols such as bisphenols, parabens, and triclosan are common environmental endocrine disruptors. Previous epidemiological studies have suggested that phenols may affect semen quality, but the results were inconsistent. In addition, most existing been limited to effects of a single chemical compound, ignoring health mixed exposure multiple chemicals. Thus, we aimed explore associations between individual various quality parameters. id="p00010">In this study, rapid sensitive method was used determine 18 phenolic compounds in urine samples 799 volunteers who donated sperm Shanghai Human Sperm Bank. A spot sample collected from each subject on day their clinic visit stored at -20 ℃ until testing. Urine (200 μL) extracted added with 20 μL an internal standard 50 β-glucuronidase solution. The mixtures then incubated for 12 h 37 ℃. After hydrolysis, twice using ethyl acetate (500 μL). concentrations measured high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Semen parameters analyzed computer-aided analyzer. Multiple linear regressions detect phenol weighted quantile sum (WQS) models mixed-phenol adjusting potential covariates, showed paraben (EtP) significantly negatively associated concentration total count (P&lt;0.05). decreased concentration; methyl (MeP) EtP identified main contributors decrease. be young males, particularly respect count.