Development and characterization of microsatellite loci for the Moroccan endemic endangered species Argania spinosa (Sapotaceae)

• DOI: 10.3732/apps.1300071
• Publication Date: 2014-04-01T12:06:23Z
• Authors: Yasmina El Bahloul; Nicolas Dauchot; Ikrame Machtoun; Fatima Gaboun; Pierre Van Cutsem
• Keywords: Sapotaceae

• Premise of the study: Microsatellite loci were developed for Moroccan endemic endangered species Argania spinosa with a combination typical library enrichment procedure and 454 GS FLX Titanium–based high‐throughput sequencing approach. Methods Results: A genomic DNA was enriched further screened using (GA) 15 , (GTA) 8 (TTC) biotin‐labeled probes coupled chemi‐luminescence detection. To increase simple sequence repeat (SSR) number, an ultra‐high‐throughput sequencing‐based approach used. Evaluation all primer pairs performed labeled dUTP on ABI 3130xl sequencer. Eleven polymorphic SSR selected out 79 regions extensively characterized 150 individuals from eight populations. Total alleles ranged six to 19 per locus while expected heterozygosity 0.618 0.869. Conclusions: The SSRs here will be used characterize genetic diversity A. across its distribution range, mainly in southern part Morocco southwestern Algeria. They may also transferable other Sapotaceae species.