Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

• DOI: 10.3748/wjg.15.2381
• Publication Date: 2009-05-20T02:35:47Z
• Authors: Min Long; Jun Luo; Yan Li; Fang-Yin Zeng; Ming Li
• Keywords: Nap; Chronic gastritis; Interleukin 8

AIM:To detect and evaluate the antibodies against Helicobacter pylori (H ) neutrophil-activating protein (HP-NAP) in patients with gastric cancer other gastroduodenal diseases. METHODS:Recombinant HP-NAP was prepared from a prokaryotic expression system Escherichia coli .Serum positivity level of HP-NAP-specific sera 43 cancer, 28 chronic gastritis, peptic ulcer, 89 healthy controls were measured by rHP-NAP-based ELISA.rHP-NAP-stimulated production interleukin-8 (IL-8) growth-related oncogene (GROα) cytokines culture supernatant SGC7901 epithelial cells also detected. RESULTS:The serum mean absorbance value c n e r g o u p ( 9 7.7 % d 1 .0 ± 0 . 2 4 w significantly higher than those gastritis group (85.7% 0.89 0.14, P < 0.005) control (27.7% 0.65 0.18, 0.001).The sensitivity specificity ELISA for detection 95.5% 91.5%, respectively.HP-NAP could slightly upregulate IL-8 cell lines but had no effect on GROα production. CONCLUSION:Infection virulent H strains secreting is associated severe diseases, may play role development carcinoma.rHP-NAPbased can be used as new method to infection.The direct limited, contribute inflammatory response or carcinogenesis activating neutrophils.