Overexpression of glucosylceramide synthase and its significance in the clinical outcome of non-small cell lung cancer

• DOI: 10.3760/cma.j.issn.0366-6999.20141331
• Publication Date: 2024-01-16T18:42:23Z
• Authors: Zhang Caiqing; Lin Xiaoyan; Song Yinghua; Zhang Xiaofang; Li Hongjia; Wang Qiangxiu
• Keywords: P-glycoprotein

Background Glucosylceramide synthase (GCS), an enzyme responsible for ceramide glycosylation, plays important role in multidrug resistance (MDR) some tumors vitro ; however, its expression and clinicopathological significance non-small cell lung cancer (NSCLC) remains unclear. Methods We evaluated GCS 116 paired tumor adjacent non-cancerous tissues 50 frozen from patients with NSCLC using immunohistochemistry western blotting, explored the correlation between characteristics prognosis. observed association MDR proteins P-glycoprotein (P-gp) resistance-related protein (LRP) to determine link at histological level. Results was significantly upregulated compared tissue. There high 75/116 specimens (64.7%) 16/116 (13.8%). High associated poor differentiation ( P =0.01), lymph node metastasis =0.004), recurrence/ distant =0.006), chemotherapy =0.025). Multivariate analysis demonstrated that immunopositivity independent risk factor survival =0.018). P-gp expressed 80/116 (69.0%) 12/116 tissue (10.3%; =0.001); LRP 85/116 (73.3%) 19/116 (16.4%; =0.001). Importantly, results increased correlated of LRP, molecules known stimulate r =0.612 0.503, =0.01 0.035, respectively). Conclusion upregulation might contribute development could be a useful prognostic indicator chemoresistance predictor patients.