In vitro effectiveness of boron neutron capture therapy for glioma cells: proliferation, apoptosis and migration

• DOI: 10.3760/cma.j.issn.1001-2346.2014.08.022
• Publication Date: 2014-08-28
• Authors: Shiwei Wang; Wan Gi Hong; Xiaoyu Yin; Zizhu Zhang; LI Jun-hua; Jie Feng; Chengkun Ye; Shuai Xu; Zhixian Gao
• Keywords: MTT assay; Neutron capture

Objective To investigate the proliferation,apoptosis and migration of boron neutron capture therapy(BNCT) for C6 rat glioma cell-line.Methods The cells,cultured by 10 B-DMEM with irradiation in-hospital irradiator (IHNI),were divided into three groups:BNCT 3 Gy (Group Ⅰ),BNCT 6 Ⅱ),untreated control lⅢ).The MTT assay was used to indentified cell proliferation draw growth curve at 1-7 days post-irradiation.The apoptosis rate detected flow cytometer (FCM) after 72 h irradiation.Cell scratch test performed post-irradiation.Results One-well number each group accounts (7.90 ± 0.23) × 105,(5.40 0.35) 105 (9.42 106.Both Group Ⅰ Ⅱ were significant difference from Ⅲ(P ＜0.01).The rates groups (37.3 0.7) ％、(84.7 2.3) ％ (23.1 1.4) ％,each showed statistic significancant others (P ＜ 0.01).Moreover,the cells decreased migrating ability irradiation.Conclusions By using therapy,it can effectively inhibit cells' proliferation,induce weaken its ability.

Key words: Boron therapy; cell; In-hospital irradiator; In vitro