Survival, differentiation and migration of the spinal cord derived neural stem cells from transgenic mice transplanted into mouse models of spinal cord injury

• DOI: 10.3760/cma.j.issn.1001-9030.2017.06.009
• Publication Date: 2017-06-08
• Authors: Jing Wang; Chunfang Wang; Xiao Li; Pengfei Li; Feng Tian; Yuantao Gao

Objective To observe the mice motion recovery and neural stem cell (NSCs) survival, migration differentiation after green fluorescent protein (GFP)+ -positive NSCs which was isolated cultivated from mouse embryonic spinal cord transplanted into models of injury lesions.

Methods mice were randomly divided control group (n=10), (n=30) (n=30). The establishing injured models. Basso-Beattie-Bresnahan (BBB) function scores, inclined plane test immunohistochemistry testing respectively evaluated recorded 1, 2, 4, 6, 8 weeks operation.

Results The hind limbs paralyzed hitting BBB scores 0. increased to 4.50 ± 1.04, 2.17 0.75, began appear differences at 2 (P=0.023). 8.17±1.47 5.33±1.03 4 (P=0.015). two groups with passage time weeks. 10.8±1.47 8.33±0.81 6 (P=0.017). difference biggest weeks, 15.50±1.37 11.17±1.16 (P=0.016). angle (17.17±3.18)°and (14.83±3.06)° 1 At (23.33±4.27)°and (18.17±2.40)°. groups’ results appeared significant in (P=0.024). (33.83±6.30)°, (23.50±1.76)°(P=0.029). small (46.83±6.05)° (36.33±7.23)°(P=0.019). can survive migrate body, some remain undifferentiated state, a part differentiate glia, less neurons.

Conclusion There promoting effect for injury. It forming micro-environment conducive resume by transplanting cells increasing proportion neurons promote recovery.

Key words: Green protein; Neural cells; Spinal injury; Cell transplantation; Mouses