[Changes in the expression of three markers in T lymphocytes of peripheral blood and immunoregulatory mechanisms of burned mice with sepsis at early stage].

• DOI: 10.3760/cma.j.issn.1009-2587.2016.02.007
• Publication Date: 2016-02-01
• Authors: Yuxiang Zhou; Peng Huang; Pihong Zhang; Lei Chen; Jizhang Zeng; Jie Zhou; Pengfei Liang; Xiaoyuan Huang
• Keywords: Expression (computer science)

To study the expression levels of annexin A1 (ANXA1), GATA-3, and T-bet in T lymphocytes peripheral blood burned mice with sepsis at early stage, to analyze their immune regulatory mechanisms.Seven-hundred eighty male clean grade were divided into sham injury group (n=60, injured on back by immersing 37 ℃ warm water for 10 s), burn (n=240, inflicted 20% TBSA deep partial- thickness 100 hot intraperitoneally injected 6 mg/kg lipopolysaccharide), burn+ (n=240) according random number table. Mice treated as that first, then they group. (1) Immediately after injury, six selected collect lymphocyte suspension (1 tube each mouse) According table, other three groups respectively post hour (PIH) 12, 24, 48, 72 collection from mouse). Each cell was equally two parts. Fluorescein isothiocyanate (FITC)-labeled human anti-mouse CD4 monoclonal antibody phycoerythrin (PE)-labeled interferon-γ added one part mark helper 1 (Th1). FITC-labeled PE-labeled interleukin-4 (IL-4) Th2. The percentages Th1 Th2 determined flow cytometer, ratio calculated. (2) 18 immediately mouse), 3 PIH mRNA ANXA1, real-time fluorescent quantitative reverse transcription-PCR. (3) 36 tubes batches (6 batch). kinds combinations: antibodies labeling combination PE-anthocyanin 7 labeled ANXA1 antibody, GATA-3 batch. time point marking surface markers above-mentioned positive cells cytometer. Data processed one-way analysis variance, variance factorial design, SNK test. relationship between Th2, level assessed linear correlation analysis.(1) Compared those significantly decreased 24 on, P values below 0.05; ratios 12 0.05. but increased 48 72, 0.05, reaching nadir (0.50±0.04, 0.45±0.03, 0.21±0.05, respectively). A significant observed (r=0.862, P<0.05). (4) cellsin lowered percentage it continuously which lower most points than since compared statistical differences (5) positively correlated (with r 0.747 0.787, 0.05).The sepsis, may play an important role Th1/Th2 balance switching bias immunosuppressive process.