Effect and safety evaluation of 50-Hz 1-mT pulsed electromagnetic field on the immunomodulation of human umbilical cord mesenchymal stem cells

• DOI: 10.3760/cma.j.issn.1673-4114.2017.02.007
• Publication Date: 2017-03-25
• Authors: 曹芳; Cao Fang; 张焕婷; Huanting Zhang; Liu Qiang; Li-qing Du; 杜利清; Liu Qiang; 曹晓沧; Xiaocang Cao
• Keywords: Comet Assay; Viability assay; Wharton's jelly

Objective To investigate the effect of 50-Hz, 1-mT pulsed electromagnetic field (PEMF) on immunomodulation human umbilical cord mesenchymal stem cells(MSCs) and to evaluate safety PEMF as a therapeutic strategy.

Methods Changes in cell viability was observed via MTT. Real-time PCR used detect expression levels cyclooxygenase 2(COX2), indoleamine 2,3-dioxygenase(IDO), IL-10, hepatocyte growth factor(HGF), transforming factor-β(TGF-β) MSCs. To explore PEMF, flow cytometry experiment conducted observe apoptosis reactive oxygen species(ROS) level. DNA damage detected comet assay. Data were statistically analyzed t-test with SPSS 19.0. P<0.05 considered significant.

Results Cell increased, most obvious increase 4 h/d(t=3.505, P<0.05). In interferon-γ (INF-γ) -activated MSC, factors improved after irradiation(t=2.436-3.747, all Furthermore, did not induce apoptosis, ROS generation, damage.

Conclusion 50-Hz, improves MSC may cause radiation injury.

Key words: Mesenchymal cell; Radiation; Pulsed field; Immunomodulation