Study on ovarian cancer cell migration induced by bisphenol A via the MAPK and PI3K signaling pathways

• DOI: 10.3760/cma.j.issn.1674-6090.2016.05.014
• Publication Date: 2016-10-25
• Authors: Xiaoqing Lu; Yu Xiao; Xiaoping Zhu; Cheng Wang

Objective To explore the role of bisphenol A in ovarian cancer cell migration and its molecular mechanism.

Methods The human OVCAR-3 line was chosen as research materials this study. The 8 μm pore size Boyden chambers QMC Chemotaxis Cell Migration Assay were adopted for analysis. molecules involved measured by real-time fluorescent quantitative PCR, western blot SensoLyte 490 kits. mechanism induced studied inhibition ERK1/2 PI3K using their corresponding inhibitors.

Results After 24 h incubation with (40 nM 100 nM) , markedly enhanced (P<0.001) . Not only gene protein expression levels MMP-2, MMP-9 N-calcium significantly increased (P<0.05, P<0.01 or P<0.001) A, but also activities MMP-2 obviously (P<0.01) indicating these three engaged caused A. Upon PI3K, invoked offset, well decreased.

Conclusion The achieved via signaling pathways.

Key words: Bisphenol A; Ovarian cancer; MAPK; PI3K; Cell