Functionalized gold nanoflowers on carbon screen-printed electrodes: an electrochemical platform for biosensing hemagglutinin protein of influenza A H1N1 virus

• DOI: 10.3762/bjnano.16.42
• Publication Date: 2025-04-16T09:37:31Z
• Authors: Carlos Enrique To...; Sharmilee Nandi; Klara Martinovic; Patrizia Kühne; Yifan Liu; Sam Taylor; Maria Lysandrou; Maria Ines Berroj...; Viktoria Langwallner; Javier Enrique Se...; Ivana Jovanovic; Maike Lüftner; Georgia-Vasiliki ...; David Bern; Abdul-Raouf Atif; Ehsan Manouchehri...; Gemma Mestres; Masood Kamali-Mog...
• Keywords: Differential pulse voltammetry; Bovine serum albumin; Surface Modification

An electrochemical biosensor based on modified carbon screen-printed electrodes was developed for the detection of hemagglutinin influenza A H1N1 virus (H1). Gold nanoflowers were electrodeposited electrode to increase conductivity and surface area. The signal amplified by functionalization gold with 4-aminothiophenol, which resulted in a 100-fold decrease charge transfer resistance due tunneling effect. Subsequently, monoclonal antibodies against H1 immobilized via covalent amide bond formation, followed blocking bovine serum albumin minimize nonspecific hydrophobic binding. characterized cyclic voltammetry impedance spectroscopy experiments presence [Fe(CN) 6 ] 3−/4− . Differential pulse used measure change current across as function concentration. This performed series samples artificial saliva containing protein clinically relevant concentration range. In these experiments, showed limit 19 pg/mL. Finally, platform coupled an automated microfluidics system, no significant observed.