[Establishment and their biological characteristics of clonal cell subpopulations (NL9980 and L9981) from a human lung large cell carcinoma cell line (WCQH-9801)].

• DOI: 10.3779/j.issn.1009-3419.2003.06.13
• Publication Date: 2003-12-20
• Authors: Qinghua Zhou; Yanping Wang; Guowei Che; Wen Zhu; Xiaohe Chen; Xiaofeng Chen; Zhilin Sun
• Keywords: clone (Java method)

To explore the possibility of separating and establishing clonal cell subpopulations with different metastatic phenotype from a human lung large carcinoma line (WCQH-9801), to identify difference biological molecular biology between NL9980 L9981 lines.Two sub-cell lines (NL9980 L9981) were isolated established (WCQH-9801) by single cloning techniques. The RELP, mRNA protein transcript expression detected in Southern blot, RT-PCR Western blot. characteristics vivo vitro determined MTT, plate, Boyden chamber methods animal models.(1)Two lines, which had phenotype, successfully (WCQH-9801). (2)The LOH nm23-H1 gene, deletion nm23-H1, but neither nor nm23-H1. (3)The proliferation, clone formation invastion significantly higher than those line. (4)The tumorigenicity rate nude mouse remarkably NL9980. (5) No significant chromosome number was observed lines.(1)NL9980 have characteristics. high invsaion metastasis ability might be related gene.