Immunolabelling Myofiber Degeneration in Muscle Biopsies

• DOI: 10.3791/59754-v
• Publication Date: 2019-12-06T00:00:09Z
• Authors: Maximilien Bencze; Baptiste Periou; Yasmine Baba-Amer; Francois J Authier
• Keywords: Pathogenesis; Immunofluorescence

The necrosis of muscle fibres (myonecrosis) plays a central role in the pathogenesis several conditions, including muscular dystrophies. Therapeutic options addressing causes dystrophy are expected to alleviate degeneration. Therefore, method assay and quantify extent cell death biopsies is needed. Conventional methods observe myofiber degeneration situ either poorly quantitative or rely on injection vital dyes. In this article, an immunofluorescence protocol described that stains necrotic myofibers by targeting immunoglobulin G (IgG) uptake myofibers. IgG based features characterizing demise, 1) loss plasma membrane integrity with release damage-associated molecular patterns 2) plasmatic proteins. murine cross-sections, co-immunolabelling myofibers, extracellular matrix proteins, mouse allows clean straightforward identification fate. This simple suitable for analysis applicable all species, human samples, does not require dye. staining can also be paired other co-immunolabelling.