Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

• DOI: 10.3791/60092
• Publication Date: 2019-10-12T00:01:09Z
• Authors: Cristina Melero; Aljona Kolmogorova; Paul Atherton; Brian Derby; Adam Reid; Karin Jansen; Christoph Ballestrem
• Keywords: Matrix (chemical analysis)

Cells sense a variety of extracellular cues, including the composition and geometry matrix, which is synthesized remodeled by cells themselves. Here, we present method Light-Induced Molecular Adsorption Proteins (LIMAP) using PRIMO system as patterning technique to produce micro-patterned matrix (ECM) substrates single or combination proteins. The enables printing ECM patterns in micron resolution with excellent reproducibility. We provide step-by-step protocol demonstrate how this can be applied study processes neuronal pathfinding. LIMAP has significant advantages over existing micro-printing methods terms ease more than one component ability generate pattern any gradient. easily adapted contribution almost chemical towards cell fate behavior. Finally, discuss common issues that arise these avoided.