Generation of Transgenic Rats using a Lentiviral Vector Approach

• DOI: 10.3791/60570
• Publication Date: 2020-05-18T00:00:24Z
• Authors: Paulina Koza; Joanna Przybyś; Agata Klejman; Gabriela Olech-Ko...; Witold Konopka
• Keywords: Transgenesis; Pronucleus; Biosafety

Transgenic animal models are fundamentally important for modern biomedical research. The incorporation of foreign genes into early mouse or rat embryos is an invaluable tool gene function analysis in living organisms. standard transgenesis method based on microinjecting DNA fragments a pronucleus fertilized oocyte. This technique widely used mice but remains relatively inefficient and technically demanding other species. transgene can also be introduced one-cell-stage via lentiviral infection, providing effective alternative to pronuclear injections, especially species strains with more challenging embryo structure. In this approach, suspension that contains vectors injected the perivitelline space embryo, which less has higher success rate. Lentiviral were shown efficiently incorporate genome determine generation stable transgenic lines. Despite some limitations (e.g., Biosafety Level 2 requirements, fragment size limits), rapid efficient method. Additionally, using female rats mated fertile male strain different dominant fur color presented as generate pseudopregnant foster mothers.